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Transformation of normal hamster cells by benzo(a)pyrene diol-epoxide

✍ Scribed by Rivka Mager; Eliezer Huberman; Shen K. Yang; Harry V. Gelboin; Leo Sachs

Publisher
John Wiley and Sons
Year
1977
Tongue
French
Weight
346 KB
Volume
19
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The frequency of cell transformation was determined after treatment of normal hamster embryo cells with benzo(a)pyrene (BP) and six of its metabolites. These metabolites included the trans 4,5‐, 7,8‐ and 9,10‐dihydrodiols; the 4,5‐epoxide; and two stereoisomers of the non‐K‐region diol epoxides r‐7, t‐8‐dihydroxy t‐9, 10‐oxy‐7,8,9,10‐tetrahydro‐benzo(a)pyrene (diol‐epoxide I) and r‐7, t‐8, dihydroxy c‐9, 10‐oxy‐7,8,9,10‐tetrahydrobenzo(a) pyrene (diol‐epoxide II). The trans 7,8‐dihydrodiol was more active than the other two dihydrodiols tested and was also more active than the parent hydrocarbon BP. Of the three epoxides tested, the diol‐epoxide I was more active than the 4,5‐epoxide and diol‐epoxide II. The results suggest that diol‐epoxide I is a major cell‐transforming metabolite of BP.

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