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Transformation of Dictyostelium with Plasmid DNA by Electroporation

✍ Scribed by Gaudet, P.; Fey, P.; Chisholm, R.

Book ID
125439356
Publisher
Cold Spring Harbor Laboratory Press
Year
2008
Tongue
English
Weight
86 KB
Volume
2008
Category
Article
ISSN
1940-3402

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Transformation of bacteria with plasmid
Transformation of bacteria with plasmid DNA by electroporation
✍ Stefan Fiedler; Reinhard Wirth πŸ“‚ Article πŸ“… 1988 πŸ› Elsevier Science 🌐 English βš– 565 KB

The possibility of electric field-mediated transformation ("electroporation") of a gram-positive bacterium (Enterococcus faecalis) and two gram-negative bacteria (Escherichia coli and Pseudomonas putida) with plasmid DNA was investigated. E. faecalis protoplasts could be transformed by electroporati