The properties of an assay for the 5-phosphoribosyl-1-pyrophosphate (PRPP) content of cultured mammalian fibroblasts are described. The assay is based upon the PRPP-dependent release of "CO, from [carboxy/-"Clorotic acid by a commercially available preparation of yeast orotidine-5'-monophosphate pyr
β¦ LIBER β¦
Transformation-associated increase of phosphoribosyl pyrophosphate concentration in chick embryo fibroblasts
β Scribed by Tapani Hovi; Antti Vaheri; Anthony C. Allison
- Book ID
- 115910392
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 400 KB
- Volume
- 103
- Category
- Article
- ISSN
- 0014-5793
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## Abstract The increase in SβadenosylβLβmethionine decarboxylase activity in chick embryo fibroblasts after infection with Rous sarcoma virus has been studied. It has been shown that enzyme levels in transformed cells were two or three times higher than those of the nonβinfected controls. The acti
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