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Transformability of DNA polymerase-deficient mutants of Bacillus subtilis

โœ Scribed by Charles T. Hadden


Book ID
118854516
Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
294 KB
Volume
51
Category
Article
ISSN
0006-291X

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The polA gene of Streptococcus pneumoniae cloned in the recombinant plasmid pSM22 is expressed in Bacillus subtilis. Extracts of B. subtilis polA mutants containing pSM22 showed 6 times more DNA polymerase activity than extracts of wild-type cells without the plasmid. Complete complementation of the