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Transferring a synthetic gene circuit from yeast to mammalian cells

✍ Scribed by Nevozhay, Dmitry; Zal, Tomasz; Balázsi, Gábor

Book ID
125472000
Publisher
Nature Publishing Group
Year
2013
Tongue
English
Weight
758 KB
Volume
4
Category
Article
ISSN
2041-1723

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Transfer of yeast artificial chromosomes
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Human DNA can he cloned as yeast artificial chromosomes (YACs), each of which contains several hundred kilobases of human DNA. This DNA can be manipulated in the yeast host using homologous recombination and yeast selectable markers. In relatively few steps it is possible to make virtually any chang