Transfer RNA genes ofEuglena gracilischloroplast DNA
β Scribed by Richard B. Hallick; Margaret J. Hollingsworth; Jac A. Nickoloff
- Book ID
- 104614260
- Publisher
- Springer
- Year
- 1984
- Tongue
- English
- Weight
- 514 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0167-4412
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β¦ Synopsis
Transfer RNA genes have been mapped to at least nine different loci on the physical map of the Euglena gracilis chloroplast genome. One of these loci in the ribosomal RNA operons is present three times per genome. The DNA sequences of six of the nine different loci, containing 21 different tRNA genes, have been determined. Genes corresponding to the amino acids Ala, Arg, Asn, Cys, Gin, Gly (2), Glu, His, lie, Leu (2), Met (2), Phe, Ser, Thr, Trp, Tyr, Val, and one unassigned species have been identified. All genes except one are found in clusters of 2-6 genes. None of the known genes contains introns, nor codes for the 3'-CCA terminus. In addition to these genes, two pseudo tRNA genes are present in the rDNA leader region.
π SIMILAR VOLUMES
Isolated chloroplasts from broad bean and common bean were found to contain a minimum of 31 and 32 tRNA species, respectively. These individual chloroplast tRNAs were 32p-labeled in vitro and hybridized to DNA fragments obtained upon digestion of broad bean and common bean chloroplast DNAs with vari