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Transfer of the E. coli O6-methyltransferase gene into repair-deficient human cells and restoration of cellular resistance to N-methyl-N′-nitro-N-nitrosoguanidine

✍ Scribed by Kanji Ishizaki; Tohru Tsujimura; Hideo Yawata; Chikau Fujio; Yusaku Nakabeppu; Mutsuo Sekiguchi; Mituo Ikenaga


Book ID
116108098
Publisher
Elsevier Science
Year
1986
Weight
527 KB
Volume
166
Category
Article
ISSN
0167-8817

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Expression of human O6-methylguanine-DNA
✍ Zining Wu; Chung-Leung Chan; Alan Eastman; Edward Bresnick 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 692 KB

We have constructed a plasmid in which the expression of human O6-methylguanine-DNA methyltransferase (MGMT) cDNA is driven by the Rous sarcoma virus promoter sequence. Transfection of this plasmid into Chinese hamster ovary (CHO) cells results in expression of MGMT and in cellular resistance to N-m