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Expression of the truncated E. coliO6-methylguanine methyltransferase gene in repair-deficient human cells and restoration of cellular resistance to alkylating agents

✍ Scribed by Kanji Ishizaki; Tohru Tsujimura; Chikau Fujio; Zhang Yangpei; Hideo Yawata; Yusaku Nakabeppu; Mutsuo Sekiguchi; Mituo Ikenaga


Book ID
116108213
Publisher
Elsevier Science
Year
1987
Weight
517 KB
Volume
184
Category
Article
ISSN
0167-8817

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Expression of human O6-methylguanine-DNA
✍ Zining Wu; Chung-Leung Chan; Alan Eastman; Edward Bresnick πŸ“‚ Article πŸ“… 1991 πŸ› John Wiley and Sons 🌐 English βš– 692 KB

We have constructed a plasmid in which the expression of human O6-methylguanine-DNA methyltransferase (MGMT) cDNA is driven by the Rous sarcoma virus promoter sequence. Transfection of this plasmid into Chinese hamster ovary (CHO) cells results in expression of MGMT and in cellular resistance to N-m