Transfer of peptide hormones from aqueous to membrane phases

✍ Scribed by Charles M. Deber; Basil A. Behnam

• Publisher: Wiley (John Wiley & Sons)
• Year: 1985
• Tongue: English
• Weight: 653 KB
• Volume: 24
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.360240110

✦ Synopsis

Peptide hormones and neurotransmitters are functional amphiphilic substances that deploy their charged and nonpolar substituents as required for traversing aqueous phases en route to their ultimate transfer into the lipid-rich environment of their membraneembedded receptors. As a means of determining the role(s) that cellular membrane lipids may play in mediating these events, we describe an experimental approach, using high-resolution 'H-and 'Gnmr spectroscopy, for delineation of the structures of complexes between the (neurotransmitter pentapeptide) enkephalins and micellar and vesicular phospholipid particles. Residue-specific enkephalin interactions with lipid are identified; affinity constants for the hydrophobic component(s) of peptide/ lipid association are calculated for enkephalin and several of its analogs; and comparisons with morphine are presented. Finally, based on molecular details obtained from nmr experiments, a model is proposed for the encounter of a peptide hormone with a phospholipid membrane surface.