Transfer of isolated nuclei into protoplasts ofSaccharomyces cerevisiae
✍ Scribed by Lajos Ferenczy; Miklós Pesti
- Publisher
- Springer
- Year
- 1982
- Tongue
- English
- Weight
- 393 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0343-8651
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
Nuclei were isolated from various types of donor protoplasts and were transferred into receptor protoplasts in numerous combinations. Five percent uptake was achieved under conditions which did not interfere with viability and subsequent culture of receptor protoplasts. Methodological investigations
When protoplasts isolated from non-respiring rho ° strains of S. cerevisiae are incubated with mitochondria from respiring rho + strains in the presence of polyethylene glycol, a small fraction of the acceptor protoplasts acquires respiratory capacity. Pretreatment of the isolated mitochondria and p
Protoplasts of a xylose-fermenting yeast strain (a fusion product of Pachysolen tannophilus and Saccharomyces cerevisiae) were fused with isolated nuclei of the xylan degrading filamentous fungus Fusarium moniliforme. Polyethyleneglycol 4OOO was used as the fusogenic agent. Fourteen stable hybrids s