A method for the efficient transfer of isolated mitochondria into yeast protoplasts

✍ Scribed by Pavol Sulo; Peter Griač; Vlasta Klobučníková; Ladislav Kováč

• Publisher: Springer-Verlag
• Year: 1989
• Tongue: English
• Weight: 551 KB
• Volume: 15
• Category: Article
• ISSN: 0172-8083
• DOI: 10.1007/bf00445745

✦ Synopsis

When protoplasts isolated from non-respiring rho ° strains of S. cerevisiae are incubated with mitochondria from respiring rho + strains in the presence of polyethylene glycol, a small fraction of the acceptor protoplasts acquires respiratory capacity. Pretreatment of the isolated mitochondria and protoplasts with Ca 2+ ions enhanced the frequency of ensuing respiration-competent colonies by almost three orders of magnitude. When mitochondria were isolated from a strain exhibiting killer capacity as a cytoplasmic marker, in addition to the appropriate nuclear and mitochondrial markers, about one-third of the ensuing rho + colonies were not killers. Possible mechanisms for the transfer of mitochondria into acceptor protoplasts were studied.