Transfer inhibition of RP4 by F factor

✍ Scribed by Tanimoto, Kouichi ;Iino, Tetsuo

• Publisher: Springer
• Year: 1983
• Tongue: English
• Weight: 590 KB
• Volume: 192
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00327654

✦ Synopsis

When RP4 and F factors were brought together into one E. coli cell, the F factor reduced 500-1000-fold the frequency of transfer of RP4. However, F had almost no effect on the surface exclusion and pilus formation by RP4. In contrast, RP4 did not affect the transfer of F. Using in vitro recombinant DNA techniques, a gene of F responsible for the above-mentioned transfer inhibition of RP4 was located within the BamHI fragment (40.4-42.8 kb) of the mini-F sequence on F. From the result of product analysis using minicells, the responsible gene in the BamHI fragment was inferred to encode the 33 K protein.