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Fertility inhibition in Rhizobium lupini by the resistance plasmid RP4

✍ Scribed by P�hler, Alfred ;Burkardt, Hans-Joachim

Publisher
Springer
Year
1978
Tongue
English
Weight
838 KB
Volume
162
Category
Article
ISSN
0026-8925

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✦ Synopsis

R plasmid RP4 inhibits the fertility of R. lupini. An RP4 carrying R. lupini donor strain is no longer capable of transferring chromosomal genes. After loss of RP4 the R. lupini fertility reappears. Plasmid RP4 spontaneously mutates at high frequency in R. lupini. RP4 mutants which do not inbibit fertility were isolated. These mutants were always transfer-defective, too. It is postulated that the genetic information for fertility inhibition in R. lupini is a substantial part of the transfer unit of the RP4 plasmid.

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Use of RP4-prime plasmids constructed in vitro to promote a polarized transfer of the chromosome in Escherichia coli and Rhizobium meliloti
✍ Julliot, Jean Simon ;Boistard, Pierre 📂 Article 📅 1979 🏛 Springer 🌐 English ⚖ 922 KB

RP4-prime plasmids containing chromosomal fragments of either Escherichia coli or Rhizobium meliloti were constructed in vitro. When introduced into E. coli or R. meliloti respectively, they promoted a polarized transfer of the chromosome as demonstrated either by the gradient of transfer of various