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Properties of plasmids constructed by the in vitro insertion of DNA fromRhizobium leguminosarum orProteus mirabilis into RP4

✍ Scribed by Jacob, A. E. ;Cresswell, Janet M. ;Hedges, R. W. ;Coetzee, J. N. ;Beringer, J. E.

Publisher: Springer
Year: 1976
Tongue: English
Weight: 885 KB
Volume: 147
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00582883

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The region of the phage lambda chromosome containing the attachment site (P.P') and the genes int and xis, excised by the action of endonuclease R.EcoRI, has been inserted into the unique site for that enzyme on the promiscuous conjugative plasmid, RP4, generating the recombinant plasmid RP4att lamb