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In vitro insertion of the ? attachment site into the plasmid RP4

✍ Scribed by Pastrana, Ricardo ;Brammar, William J.

Publisher: Springer
Year: 1979
Tongue: English
Weight: 575 KB
Volume: 177
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00267266

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✦ Synopsis

The region of the phage lambda chromosome containing the attachment site (P.P') and the genes int and xis, excised by the action of endonuclease R.EcoRI, has been inserted into the unique site for that enzyme on the promiscuous conjugative plasmid, RP4, generating the recombinant plasmid RP4att lambda. Transformants containing the hybrid plasmid were recognised by their ability to allow efficient lysogenization by phage lambda b2 (Weil and Signer, 1968; Echols et al., 1968) containing the mutant attachment site delta. P'. The construction and properties of the hybrid plasmid RP4att lambda are described.

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