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Transfection of PC12 cells with the human GAP-43 gene: effects on neurite outgrowth and regeneration

✍ Scribed by Yankner, Bruce A.; Benowitz, Larry I.; Villa-Komaroff, Lydia; Neve, Rachael L.

Book ID
123332779
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
906 KB
Volume
7
Category
Article
ISSN
0169-328X

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✍ E. R. A. Jap Tjoen San; M. H. Schmidt-Michels; B. M. Spruijt; A. B. Oestreicher; πŸ“‚ Article πŸ“… 1991 πŸ› John Wiley and Sons 🌐 English βš– 546 KB

A combined assay to measure neurite outgrowth and B-50/GAP-43 levels in PC12 cells is reported. During NGF-induced neuritogenesis, B-50/GAP-43 expression was monitored by enzyme-linked immunosorbent assay (ELISA). Neurite outgrowth was quantified at the same time by the use of video image analysis.