✦ LIBER ✦

Quantitation of the growth-associated protein B-50/GAP-43 and neurite outgrowth in PC12 cells

✍ Scribed by E. R. A. Jap Tjoen San; M. H. Schmidt-Michels; B. M. Spruijt; A. B. Oestreicher; Dr. P. Schotman; W. H. Gispen

Publisher: John Wiley and Sons
Year: 1991
Tongue: English
Weight: 546 KB
Volume: 29
Category: Article
ISSN: 0360-4012
DOI: 10.1002/jnr.490290203

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✦ Synopsis

A combined assay to measure neurite outgrowth and B-50/GAP-43 levels in PC12 cells is reported. During NGF-induced neuritogenesis, B-50/GAP-43 expression was monitored by enzyme-linked immunosorbent assay (ELISA). Neurite outgrowth was quantified at the same time by the use of video image analysis. Sensitivity and reliability of the methods are shown with a dose-response and time curve of p-NGF-induced neuritogenesis. A linear increase in total length of neurites was induced by concentrations of p-NGF 2 5 nglrnl and was accompanied by a linear increase in the amount of B-501GAP-43. The combined methods presented here can conveniently and reliably establish subtle changes in neurite outgrowth and intracellular protein contents.

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