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Transfection of MCF-7 Carcinoma Cells with Human Integrin α7 cDNA Promotes Adhesion to Laminin

✍ Scribed by Ioannis S. Vizirianakis; Chung-Chen Yao; YaoQi Chen; Barry L. Ziober; Asterios S. Tsiftsoglou; Randall H. Kramer


Book ID
115568536
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
329 KB
Volume
385
Category
Article
ISSN
0003-9861

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Cytofluorimetric and reverse-transcription polymerase chain reaction (RT-PCR) analysis showed that adriamycinresistant (ADR R ), but not sensitive (WT), MCF-7 human mammary carcinoma cell lines express a4b1 and a5b1 integrins. ADR R cells adhere to fibronectin (FN), and only a5b1 is involved in cell