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Transcriptional regulatory code of a eukaryotic genome

✍ Scribed by Harbison, Christopher T.; Gordon, D. Benjamin; Lee, Tong Ihn; Rinaldi, Nicola J.; Macisaac, Kenzie D.; Danford, Timothy W.; Hannett, Nancy M.; Tagne, Jean-Bosco; Reynolds, David B.; Yoo, Jane


Book ID
109893150
Publisher
Nature Publishing Group
Year
2004
Tongue
English
Weight
393 KB
Volume
431
Category
Article
ISSN
0028-0836

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Cloning of a eukaryotic regulatory gene
✍ Losson, RΓ©gine ;Lacroute, FranΓ§ois πŸ“‚ Article πŸ“… 1981 πŸ› Springer 🌐 English βš– 754 KB

From a pool of hybrid plasmids carrying Sau3A fragments representing the entire yeast (S. cerevisiae) genome, a DNA fragment containing the regulatory gene PPRI was cloned by complementation of a non-inducible pprl mutation which confers to the cells an increased sensitivity to 6-azauracil. Cells co