Transcription terminators in lambda appendix Deletion of control sites in the pL operon of phage lambda
β Scribed by H. Inokuchi; W.F. Dove
- Book ID
- 119009708
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 143 KB
- Volume
- 88
- Category
- Article
- ISSN
- 0042-6822
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π SIMILAR VOLUMES
A bacteriophage lambda cloning vector carrying the trp/lacW205 substitution is described. The vector facilitates the fusion in vitro of genetic control signals to the lacZ structural gene of Escherichia coli. This system was used to define transcriptional termination sites in the lambda b2 region. T
RNA polymerase binds very tightly at a site called Brex in the lambda immunity region, to the left of the rex gene and about 600 nucleotides to the right of PL. The complex formed is resistant to 1 M NaCl in the absence of nucleotide triphosphate. While in vitro little or no transcription is observe