Lambda phages carrying the R100 finO gene have been isolated from an R100:: lambda cointegrate in which lambda was inserted into the R100 traD gene at kb coordinate 72.1. Physical analyses of these phages place the finO gene within R100 SalI fragment D, near kb coordinate 82.0. Analysis of proteins
Transcription of the Transfer Genes traY and traM of the Antibiotic Resistance Plasmid R100-1 Is Linked
β Scribed by David Stockwell; Vera Lelianova; Teresa Thompson; Walter B Dempsey
- Book ID
- 112263503
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 142 KB
- Volume
- 43
- Category
- Article
- ISSN
- 0147-619X
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The traJ gene and the 5' end of the neighbouring traY gene of the resistance plasmid R1 were sequenced. Both structural genes show relatively little homology with the corresponding sequences of the related F plasmid. At the amino acid level sufficient homology is detected to allow an assignment of t
We have cloned the EcoRI fragments of pLC1, a circular DNA element found in an Escherichia coli dnaAts strain integratively suppressed by R100.1 (Chandler et al., 1977a), using the plasmid vector pCR1. All the resistance genes known to be present on the r-determinant of R100.1 were found to be prese