We have cloned the EcoRI fragments of pLC1, a circular DNA element found in an Escherichia coli dnaAts strain integratively suppressed by R100.1 (Chandler et al., 1977a), using the plasmid vector pCR1. All the resistance genes known to be present on the r-determinant of R100.1 were found to be prese
The finO gene of antibiotic resistance plasmid R100
β Scribed by Dempsey, Walter B. ;McIntire, Sarah A.
- Publisher
- Springer
- Year
- 1983
- Tongue
- English
- Weight
- 876 KB
- Volume
- 190
- Category
- Article
- ISSN
- 0026-8925
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β¦ Synopsis
Lambda phages carrying the R100 finO gene have been isolated from an R100:: lambda cointegrate in which lambda was inserted into the R100 traD gene at kb coordinate 72.1. Physical analyses of these phages place the finO gene within R100 SalI fragment D, near kb coordinate 82.0. Analysis of proteins synthesized by the phages did not identify the finO gene product, although a constitutive protein of m.w. 30,100 was encoded by R100 DNA between kb coordinates 78.7 and 81.2.
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