Transcriptional properties of an "albino" mutant, a "slime" mutant and a wild type strain of Neurospora crassa have been compared by quantitative estimates of DNA:RNA hybridization. The RNAs isolated from these strains were incubated with 32P-DNA of the wild type strain. The DNA:RNA hybrids were iso
Transcription of repetitive DNA in Neurospora crassa
โ Scribed by Dutta, S. K. ;Chaudhuri, R. K.
- Publisher
- Springer
- Year
- 1975
- Tongue
- English
- Weight
- 409 KB
- Volume
- 136
- Category
- Article
- ISSN
- 0026-8925
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โฆ Synopsis
Repeated DNA sequences of Neurospora crassa were isolated and characterized. Approximately 10-12% of N. crassa DNA sequence were repeated, of which 7.3% were found to be transcribed in mid-log phase of mycelial growth as measured by DNA:RNA hybridization. It is suggested that part of repetitive DNA transcripts in N. crassa were mitochondrial and part were nuclear DNA. Most of the nuclear repeated DNAs, however, code for rRNA and tRNA in N. crassa.
๐ SIMILAR VOLUMES
The genetic segregation of ribosomal DNA (rDNA) in Neurospora crassa was analyzed by exploiting restriction fragment length polymorphisms in the nontranscribed spacer (NTS) sequences of nine laboratory wild-type strains and wild-collected strains. In an analysis of random spore progeny from seven cr