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Ribosomal DNA inheritance and recombination in Neurospora crassa

✍ Scribed by Russell, Peter J. ;Petersen, Richard C. ;Wagner, Sheryl


Publisher
Springer
Year
1988
Tongue
English
Weight
340 KB
Volume
211
Category
Article
ISSN
0026-8925

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✦ Synopsis


The genetic segregation of ribosomal DNA (rDNA) in Neurospora crassa was analyzed by exploiting restriction fragment length polymorphisms in the nontranscribed spacer (NTS) sequences of nine laboratory wild-type strains and wild-collected strains. In an analysis of random spore progeny from seven crosses, and of ordered tetrads from two of those crosses the rDNA was shown to be inherited in a simple, stable Mendelian fashion, exhibiting an approximately 1 : 1 ratio of the two parental rDNA types. No meiotic recombinants were detected among the progeny, indicating that non-sister-chromatid crossing over is highly suppressed in the rDNA region. The basis for this suppression of meiotic recombination is not known.


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