Trace determination of sugar acids (gluconic acid) in sea water by liquid chromatography

✍ Scribed by Shigeto Nakabayashi; Isao Kudo; Kenshi Kuma; Katsuhiko Matsunaga; Hasebe Kiyoshi

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 367 KB
• Volume: 271
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)80547-x

✦ Synopsis

A hqmd chromatographlc (LC) method wth fluorescence detection was developed mth sufficient sensltlvlty to deternune organic acids m sea water and sedunents Sugar acids, except for uromc and a-keto acids, have not previously been measured III sea water because of then low concentrations After desalting wth a column, glucomc acid was esterLfied with 9-anthryldmxomethane The reaction muture contammg the glucomc acid denvatlve was directly chromatographed by LC mung an octadecylsllane reversed-phase column and fluorescence detection The peak areas were hnearly related to glucomc acid concentration The detetion limit was less than 10 nM The reproduabhty and recovery were 8% at 200 nM glucomc acid and 99 8%, respectively kYeywora3 Fluonmetry, bqmd chromatography, Glucomc acid, Sea water, Sugar acids, Waters