TPA-enhanced invasion of matrigel associated with augmentation of cell motility but not metalloproteinase activity in a highly metastatic variant (L-10) of human rectal adenocarcinoma cell line RCM-1

✍ Scribed by Kazuki Nabeshima; Naoto Komada; Jun-Ichi Kishi; Hiroyuki Koita; Teruhiko Inoue; Taro Hayakawa; Masashi Koono

• Book ID: 102864436
• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: French
• Weight: 952 KB
• Volume: 55
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910550617

✦ Synopsis

We previously found that the enhanced activity to invade Matrigel upon stimulation with 12-0-tetradecanoylphorbol-13acetate (TPA) was one of the major properties of a highly metastatic variant (L-10) of a human rectal adenocarcinoma cell line RCM-I. To clarify the mechanism of this enhancement, we examined the effect of TPA on 2 major biological factors involved in tumor cell invasion: cell motility and matrixdegrading metalloproteinase activity. The enhanced invasiveness was inhibited by protein-kinase-C inhibitors. TPA markedly enhanced both haptotactic response to type-IV collagen and motility on tissue-culture glass substrate of L-I0 cells in a 3To whom correspondence and reprint requests should be addressed.