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Toxoplasma gondii: A simple high-throughput assay for drug screening in vitro

✍ Scribed by ChunMei Jin; Kusuma Kaewintajuk; JingHua Jiang; WooJin Jeong; Masaki Kamata; Hye-Sook KIM; Yusuke Wataya; Hyun Park


Book ID
113617705
Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
351 KB
Volume
121
Category
Article
ISSN
0014-4894

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We developed a 96-well microtiter-plate highthroughput screening (HTS) assay for the detection of modulators of transcription. This HTS assay consists of three steps: (1) the in vitro transcription reaction; (2) modification and hybridization of RNA products; and (3) washing and quantification. Dur