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Toxic effects of ouabain and food and cosmetic dyes on nerve growth factor-promoted differentiation of neurites in culture

✍ Scribed by Stephen J. Morris; Bibie M. Chronwall

Publisher: John Wiley and Sons
Year: 1982
Tongue: English
Weight: 607 KB
Volume: 7
Category: Article
ISSN: 0360-4012
DOI: 10.1002/jnr.490070311

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The toxic effects of ouabain and various fluorescein analogs have been tested on nerve growth factor‐promoted (NGF‐promoted) outgrowth of neurites from embryonic chick sensory ganglia embedded in a matrix of native collagen. Ouabain inhibits outgrowth with an IC~50~ of approximately 5 μM. Erythrosin B (USFD&C Red 3) and other fluorescein derivative dyes brominated or iodinated on the xanthene ring also inhibit neurite outgrowth with IC~50~'S as low as 10 μm and promote early retraction of growing neurites. The effects of the dyes are due, at least in part, to photooxidation of the NGF, but they may also act directly on the cells. The culture system should prove useful as both a general and a specific in vitro assay for neurotoxicity.

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