Towards proteome scale antibody selections using phage display
✍ Scribed by Michael Mersmann; Doris Meier; Jana Mersmann; Saskia Helmsing; Peter Nilsson; Susanne Gräslund; Structural Genomics Consortium; Karen Colwill; Michael Hust; Stefan Dübel
- Book ID
- 108218414
- Publisher
- Elsevier
- Year
- 2010
- Tongue
- English
- Weight
- 919 KB
- Volume
- 27
- Category
- Article
- ISSN
- 1871-6784
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Phage antibody-display is rapidly maturing into a very effective tool for antibody generation. The recent development of large primary antibody libraries enables selection of antibodies against most targets in under two weeks and many of these antibodies have relatively high (nanomolar) affinities.
The generation of new antibodies for diagnostic applications using phage display could greatly decrease the time and expense of new assay development but, to be effective, the process must yield antibodies with desired specificity and affinity comparable to those obtained by monoclonal antibody tech