Toward a Mechanism-Based Fluorescent Assay for Site-Specific Recombinases and Topoisomerases: Assay Design and Syntheses of Fluorescent Substrates
β Scribed by Panigrahi, Gagan; Zhao, Bao-ping; Krepinsky, Jiri J.; Sadowski, Paul D.
- Book ID
- 127141328
- Publisher
- American Chemical Society
- Year
- 1996
- Tongue
- English
- Weight
- 543 KB
- Volume
- 118
- Category
- Article
- ISSN
- 0002-7863
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A fluorescent water-soluble substrate for phosphatidylinositol-specific phospholipase C was synthesized. The diacylglycerol moiety of the natural substrate, phosphatidylinositol, was replaced by the fluorescent moiety, 2-naphthol, resulting in the synthetic substrate, racemic 2-naphthyl myo-inositol