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Total bile acids in hyperlipidaemic serum determined by bioluminescent and spectrophotometric methods

✍ Scribed by Lekhakula, Somsong ;Boonpisit, Sasiprapa ;Amornkitticharoen, Boonthum

Publisher: John Wiley and Sons
Year: 1991
Weight: 272 KB
Volume: 6
Category: Article
ISSN: 0884-3996
DOI: 10.1002/bio.1170060408

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✦ Synopsis

Abstract

A simple, rapid and sensitive bioluminescent method has been used to measure total bile acids in hyperlipidaemic serum. We found that the levels of total bile acids in hypertriglyceridaemic and hypercholesterolemic sera determined by a spectrophotometric method were four‐fold higher than those measured by the bioluminescent method (6.73 ± 4.07 μmol/l (mean ± SD) by bioluminescent and 26.10 ± 13.42 μmol/l by the spectrophotometric method). There was no difference in total bile acid levels between these two methods for normal serum (4.72 ± 3.38 μmol/l by bioluminescence and 4.49 ± 3.27 μmol/l by the spectrophotometric method).

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