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Toroidal nucleoids in Escherichia coli exposed to chloramphenicol

โœ Scribed by Steven B. Zimmerman


Book ID
117704430
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
417 KB
Volume
138
Category
Article
ISSN
1047-8477

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Membrane-attached nucleoids were isolated from E. coli and separated from proteins by 2 M NaCl. Dcsintegration of such nucleoids by ultrasound and subsequent centrifugation resulted in the formation of two fractions: a sediment (fraction I) and a supernatant (fraction 11). The protein:DNA ratio of