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Multiple Restraints to the Unfolding of Spermidine Nucleoids from Escherichia coli

โœ Scribed by Lizabeth D Murphy; Steven B Zimmerman


Book ID
115634158
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
382 KB
Volume
132
Category
Article
ISSN
1047-8477

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Membrane-attached nucleoids were isolated from E. coli and separated from proteins by 2 M NaCl. Dcsintegration of such nucleoids by ultrasound and subsequent centrifugation resulted in the formation of two fractions: a sediment (fraction I) and a supernatant (fraction 11). The protein:DNA ratio of