Topological distribution of virus-specific and cross-reactive antigenic determinants on the gB glycoprotein of the herpes simplex viruses

✍ Scribed by R. Eberle; R. J. Courtney

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 875 KB
• Volume: 27
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/jmv.1890270410

✦ Synopsis

The antigenic properties and relations between the herpes simplex virus type 1 and 2 (HSV1, HSVZ) gB glycoproteins were investigated. Using several assay systems to analyze the virusspecific reactivity of polyclonal monospecific rabbit anti-gB sera, it was demonstrated that most of the antigenic determinants of the gB g\ycoproteins exposed at the surface of both virions and infected cells are virus-specific rather than cross-reactive. Comparative examination of the reactivity of human immune sera with HSVl and HSV2 antigens by immunoblotting also revealed differences in the ability of HSVI and HSV2 immune sera to recognize homologous vs. heterologous gB antigens. These results indicate that despite the high degree of amino acid sequence homology which exists between the HSVl and HSV2 gE glycoproteins many of the imrnunologically relevant antigenic determinants of the gB glycoproteins probably reside in regions of the motecule which are not so highfy canserved between the two HSV serotypes.