Tn10 insertional mutations of Bacillus subtilis that block the biosynthesis of bacilysin
✍ Scribed by Ayten Yazgan; Gülay Özcengiz; Mohamed A. Marahiel
- Book ID
- 114147315
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 377 KB
- Volume
- 1518
- Category
- Article
- ISSN
- 0167-4781
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Exploitation of the ability of the ban protein encoded by phage P1 to compensate for dnaB-defective host mutations, allowed the isolation of dnaB: :Tnl0 insertion mutations. The presence of Plbac prophage was required for survival of dnaB::TnlO mutants, and such lysogens were cryosensitive. The inse
Three new genes nifM, nifI and nifN have been mapped in the nif gene cluster of Klebsiella pneumoniae and a fourth gene nifJ has been confirmed as being a separate cistron. Polar nif mutations were obtained by transposition of Tn7 to plasmid pRD1, and of Tn5 and Tn10 to plasmid pMF100, a derivative