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The isolation and characterization of Escherichia coli dnaB::Tn10 insertion mutations

✍ Scribed by Sclafani, Robert A. ;Wechsler, James A. ;Schuster, Heinz

Publisher: Springer
Year: 1981
Tongue: English
Weight: 737 KB
Volume: 182
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00422776

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✦ Synopsis

Exploitation of the ability of the ban protein encoded by phage P1 to compensate for dnaB-defective host mutations, allowed the isolation of dnaB: :Tnl0 insertion mutations. The presence of Plbac prophage was required for survival of dnaB::TnlO mutants, and such lysogens were cryosensitive. The insertions were shown to map in dnaB by transduction and this was confirmed by complementation analysis. The dnaB::TnlO (P1 bac) strains were non-permissive for 2 growth but did support the growth of 2-dnaB + specialized transducing phage. No antigenically active dnaB product could be detected by immunologic assays using either of two methods. In addition, it was shown that the observed cryosensitivity of Plbac suppression was a direct result of reversible inactivation of the ban protein at low temperature.

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