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Transfer of the Δ (argF-lac) U169 mutation between Escherichia coli strains by selection for a closely linked Tn10 insertion

✍ Scribed by Schweizer, Herbert ;Boos, Winfried

Publisher: Springer
Year: 1983
Tongue: English
Weight: 200 KB
Volume: 192
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00327683

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✦ Synopsis

To facilitate construction of mutants harboring delta lac for use in gene fusion studies, strains were constructed that carry the transposon Tn10 next to the well defined lac deletion U169. This deletion can now be moved to other Escherichia coli strains in transductional or conjugational crosses by selecting resistance to tetracycline.