TLC of coumarin anticoagulants

✍ Scribed by C. A. Lau-Cam; I. Chu-Fong

• Publisher: John Wiley and Sons
• Year: 1972
• Tongue: English
• Weight: 435 KB
• Volume: 61
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600610828

✦ Synopsis

Product mg. TCEb Method Independent Method ~ A 100 115.13 =I= 2.78 111.81 f 1 1 . 3 F B 100 97.77 =k 5.36 102.98 + 6.3& C 75 77.98 f 2.78 79.32 =I= 2 . 9 9 ~ D 75 76.32 f 2.09 80.74 =I= 2.65" E 5015 ml. 54.10 f 0.44 53.81 f 0.20d F 60/0.6 ml. 61.41 0 . 5 2 62.00 =I= 0.21d Calculated on basis of five doses; each assayed by TCE and independent method. b Tubular carbon electrode method. c Reference 8. d Re.ferences 9 and IO.

Several compounds commonly used as antioxidants undergo oxidation at carbon electrodes ( 7), but no interference by these compounds with the ascorbic acid assay is expected for two reasons:

1. The concentrations of antioxidants is usvally low and will not be detectable in the samples diluted (usually 1 : 10,000) for analysis.