Tissue-specific differences in adduct formation by hepatocarcinogenic and sarcomatogenic derivatives of 7H-dibenzo[c,g]carbazole in mouse parenchymal and nonparenchymal liver cells

✍ Scribed by Odette Périn-Roussel; Nicole Barat; François Zajdela; François Périn

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 330 KB
• Volume: 29
• Category: Article
• ISSN: 0893-6692
• DOI: 10.1002/(sici)1098-2280(1997)29:4<346::aid-em2>3.0.co;2-8

✦ Synopsis

Parenchymal (PC) and nonparenchymal (NPC) liver which is exclusively hepatocarcinogenic, were anacells have different tissue-specific, procarcinogen lyzed in parallel. Both PC and NPC generated the activation enzymes. NPC appear to be protected ultimate metabolites of DBC, but NPC were substanagainst the mutagenic effects of lipotropic bulky ad-tially less efficient. Clear-cut tissue-specific differducts induced by carcinogens by a unknown mech-ences in adduct formation were established: the saranism. Most studies of activation have been con-comagenic 6MeODBC gave rise only to NPC-DNA ducted with whole liver. The purpose of this study adducts, and the hepatocarcinogenic DiMeDBC was to differentiate adduct formation in mouse PC only to PC-DNA adducts. The chromatograms of and in NPC, isolated after in vivo administration of the adducts were compared with those of mouse 7H-dibenzo[c,g]carbazole (DBC), the most efficient embryonic cells in culture and mouse epidermal liver carcinogen in mice, which also has potent sar-cells. The results are discussed in connection with comagenic and epitheliomagenic activities. The animal experiments with DBC, 6MeODBC, and divery sensitive 32 P-postlabeling method was used to methylbenzanthracene and with published data on detect adducts. Two tissue-specific DBC derivatives, PC and NPC activating enzymes. Environ. Mol. Mu-6-methoxy-DBC (6MeODBC), which is exclusively tagen. 29:346-356, 1997.