DNA adduct formation in primary mouse embryo cells induced by 7H-dibenzo[c,g]carbazole and its organ-specific carcinogenic derivatives

✍ Scribed by A. Gábelová; O. Périn-Roussel; Y. Jounaidi; F. Périn

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 157 KB
• Volume: 30
• Category: Article
• ISSN: 0893-6692
• DOI: 10.1002/(sici)1098-2280(1997)30:1<56::aid-em8>3.0.co;2-a

✦ Synopsis

The nuclease P1 modification of the 32 P-postlabeling DNA-adducts in the embryo cells, which suggests that technique was used to study the biological activity the enzymatic composition of the target tissue probaof 7H-dibenzo[c,g]carbazole (DBC) and some of its bly is the determining factor in the organ specificity of derivatives, including N-methyldibenzo[c,g]carbazole this derivative. 5,9,N-triMeDBC, derivative without (N-MeDBC), 5,9-dimethyldibenzo[c,g]carbazole (5,9-any carcinogenic activity in vivo, did not induce any diMeDBC), 5,9,N-trimethyldibenzo[c,g]carbazole DNA-adducts in primary mouse embryo cells. Pretreat-(5,9,N-triMeDBC), 6-methoxydibenzo[c,g]carbazole ment of cells with 2,3,7,8-tetrachlorodibenzo-p-dioxin (6-McODBC), N-acetyldibenzo[c,g]carbazole (N-(TCDD) apparently stimulated DNA-adduct forma-AcDBC), N-hydroxymethyldibenzo[c,g]carbazole (N-tion in the cells exposed to DBC, 6-MeODBC, and HMeDBC) in primary mouse embryo cells. A very N-MeDBC. No or a very slight effect of TCDD on good correlation was found between carcinogenic DNA-adduct formation was found in cells exposed specificity in vivo of these N-heterocyclic aromatic hy-to N-HMeDBC and N-AcDBC. Preliminary results drocarbons and their DNA-adduction in vitro. Primary have shown that TCDD slightly induced cytochrome mouse embryo cells were able to metabolize and de-P4501A1-linked ethoxyresorufin O-deethylase (EROD) tect tissue-specific sarcomagens N-MeDBC and 6-activity in primary mouse embryo cells. These data MeODBC as well as derivatives with both sarco-suggest the role of cytochrome P4501A1 in the memagenic and hepatocarcinogenic activity, DBC, N-tabolism of DBC derivatives with sarcomagenic AcDBC, and N-HMeDBC. The strong specific hepato-activity.