Time-resolved polarization measurements of porphyrin fluorescence in solution and in single cells

✍ Scribed by H.K. Seidlitz; H. Schneckenburger; K. Stettmaier

• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 374 KB
• Volume: 5
• Category: Article
• ISSN: 1011-1344
• DOI: 10.1016/1011-1344(90)85053-y

✦ Synopsis

Porphyrin monomers, dimers and aggregates, which can be differentiated on the basis of their fluorescence lifetimes, are shown to possess different degrees of fluorescence polarization. This opens up new possibilities for microscopic imaging of these individual components in photosensitization and tumour detection. A rough estimate of the size of the porphyrin aggregates is obtained from the data of time-resolved fluorescence anisotropy.