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Time-resolved fluorescence study of human recombinant interferon α2 : Association state of the protein, spatial proximity of the two tryptophan residues

✍ Scribed by Michel VINCENT; Ines M. LI DE LA SIERRA; Mário N. BERBERAN-SANTOS; Aída DIAZ; Maylin DIAZ; Gabriel PADRON; Jacques GALLAY


Book ID
115129768
Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
835 KB
Volume
210
Category
Article
ISSN
1432-1327

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Interaction of recombinant human epiderm
✍ Ines M. Sierra; Michel Vincent; Gabriel Padron; Jacques Gallay 📂 Article 📅 1992 🏛 Springer 🌐 English ⚖ 794 KB

The interaction of recombinant human epidermal growth factor with small unilamellar phospholipid vesicles was studied by steady-state and time-resolved fluorescence of the bis-tryptophan sequence (Trp49-Trp50). Steady-state anisotropy measurements demonstrate that strong binding occurred with small