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Protein flexibility and aggregation state of human epidermal growth factor : A time-resolved fluorescence study of the native protein and engineered single-tryptophan mutants

✍ Scribed by Jacques GALLAY; Michel VINCENT; Ines M. Li de la SIERRA; Julio ALVAREZ; Raimundo UBIETA; Juan MADRAZO; Gabriel PADRON


Book ID
115130451
Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
700 KB
Volume
211
Category
Article
ISSN
1432-1327

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Interaction of recombinant human epiderm
✍ Ines M. Sierra; Michel Vincent; Gabriel Padron; Jacques Gallay πŸ“‚ Article πŸ“… 1992 πŸ› Springer 🌐 English βš– 794 KB

The interaction of recombinant human epidermal growth factor with small unilamellar phospholipid vesicles was studied by steady-state and time-resolved fluorescence of the bis-tryptophan sequence (Trp49-Trp50). Steady-state anisotropy measurements demonstrate that strong binding occurred with small