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Time-resolved fluorescence and absorption microspectroscopy of a single microparticle

✍ Scribed by Hiroshi Masuhara; Keiji Sasaki

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
927 KB
Volume
299
Category
Article
ISSN
0003-2670

No coin nor oath required. For personal study only.

✦ Synopsis

Time-resolved fluorescence and transient absorption spectroscopy systems have been developed for elucidating photophysical and photochemical processes occurring in pm-sized volumes. The fluorescence measurement is based on a confocal fluorescence microscope and single photon timing, which enables the fluorescence dynamics to be observed with both sub+m threedimensional space-and ps time resolutions. For transient absorption spectroscopy, excitation and monitoring laser pulses are coaxially and confocally introduced into a microscope. This optical arrangement provides the longitudinal resolution in addition to the lateral resolution. By combining a laser trapping technique we have succeeded in measuring primary photoprocesses of a single manipulated particle in solution. Fluorescence analysis of individual microcapsules in solution gave information on solute concentration distribution between capsules. The viscosity of a single oil droplet dispersed in aqueous solution was estimated by analyzing the T-T annihilation of Zn-tetraphenylporphyrin.

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