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Time-dependentAluI action on human chromosomes

✍ Scribed by A. Ma. Fernández-Peralta; I. Tagarro; P. Ludeña Reyes; C. Sentís Castaño; J. Fernández Piqueras; J. J. González-Aguilera

Publisher
Springer Netherlands
Year
1991
Tongue
English
Weight
775 KB
Volume
84
Category
Article
ISSN
0016-6707

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✦ Synopsis

We have analyzed the pattern of A/u1 digestion over time on human chromosomes in order to monitor the evolution of the in situ enzyme action. Short treatments followed by Giemsa staining produce a G-like banding effect, whereas longer treatments produce a C-like banding pattern. However, when Propidium iodide staining is used, it reveals a uniform bright fluorescence after short Ah1 digestions and C bands when longer treatments are developed. We propose that C banding is the result of a uniform DNA removal in non centromeric regions taking place after a critical time point, the initial G like banding being produced by changes in the DNA-proteins interactions.

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