𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Thymol and carvacrol binding to DNA: model for DRUG–DNA interaction

✍ Scribed by Sh. Nafisi; A. Hajiakhoondi; A. Yektadoost

Publisher
Wiley (John Wiley & Sons)
Year
2004
Tongue
English
Weight
170 KB
Volume
74
Category
Article
ISSN
0006-3525

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Thymol and carvacrol can bind to major and minor grooves of B‐DNA. The aim of this study was to examine the interaction of calf thymus DNA with thymol and carvacrol in aqueous solution and physiological pH with thymol/DNA and carvacrol/DNA (phosphate) molar ratios of 1/20, 1/10, 1/5, and 1/1. Fourier transform infrared and UV‐visible difference spectroscopy were used to determine the thymol and carvacrol binding mode, binding constant, sequence selectivity, DNA secondary structure, and structural variations of thymol/DNA and carvacrol/DNA complexes in aqueous solution. Spectroscopic evidence showed that the thymol and carvacrol interaction occurred mainly through H‐bonding of the thymol and carvacrol OH group to the guanine N7, cytosine N3, and backbone phosphate group with overall binding constant of K(thymol–DNA) = 2.43 × 10^3^M^−1^, K(carvacrol–DNA) = 1.55×10^3^M^−1^. In thymol and carvacrol–DNA complexes, DNA remains in the B‐family structure. © 2004 Wiley Periodicals, Inc. Biopolymers, 2004

📜 SIMILAR VOLUMES

Tight Binding of the Antitumor Drug Dite
Tight Binding of the Antitumor Drug Ditercalinium to Quadruplex DNA
✍ Carolina Carrasco; Frédéric Rosu; Valérie Gabelica; Claude Houssier; Edwin De Pa 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 242 KB

The structural selectivity of the DNA-binding antitumor drug ditercalinium was investigated by competition dialysis with a series of nineteen different DNA substrates. The 7H-pyridocarbazole dimer was found to bind to double-stranded DNA with a preference for GC-rich species but can in addition form

3′-Azido-3′-deoxythymidine binding to ri
3′-Azido-3′-deoxythymidine binding to ribonuclease A: Model for drug-protein interaction
✍ S. Gaudreau; A. Novetta-Dellen; J. F. Neault; S. Diamantoglou; H. A. Tajmir-Riah 📂 Article 📅 2003 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 123 KB

## Abstract Ribonuclease A (RNase A) with several high affinity binding sites is a possible target for many organic and inorganic molecules. 3′‐Azido‐3′‐deoxythymidine (AZT) is the first clinically effective drug for the treatment of human immunodeficiency virus (HIV) infection. The drug interactio

A structural model for the rolA protein
A structural model for the rolA protein and its interaction with DNA
✍ D.J. Rigden; M. Carneiro 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 288 KB 👁 2 views

The study of the plant oncogene rolA has been hampered by a lack of structural information. Here we show that, despite a lack of significant sequence similarity to proteins of known structure, the rolA sequence adopts a known fold; that of the papillomavirus E2 DNA-binding domain. This fold is relia

Water regulation of actinomycin-D bindin
Water regulation of actinomycin-D binding to DNA: The interplay among drug affinity, DNA long-range conformation, and hydration
✍ João Ruggiero Neto; Marcio Francisco Colombo 📂 Article 📅 2000 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 143 KB 👁 2 views

Actinomycin-D (actD) binds to natural DNA at two different classes of binding sites, weak and strong. The affinity for these sites is highly dependent on DNA sequence and solution conditions, and the interaction appears to be purely entropic driven. Although the entropic character of this reaction h