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Thrombin-induced adherence of neutrophils to cultured endothelial monolayers: Increased endothelial adhesiveness

✍ Scribed by Rena Bizios; Linda C. Lai; Jeffrey A. Cooper; Peter J. Del Vecchio; Asrar B. Malik

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
687 KB
Volume
134
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

We examined the effects of a-thrombin on the adherence of neutrophils to endothelial cell monolayers. Endothelial cells derived from the ovine pulmonary artery and ovine neutrophils were used. Thrombin M) resulted in a time-dependent increase in neutrophil adherence to the endothelium. The response was concentration-dependent with a maximal response at M. Thrombin did not induce neutrophil adherence either to plastic or to endothelial cell-derived matrix. The adherence response was inhibited in the presence of a-thrombin that had been inactivated with anti-thrombin Ill (1U:lU) or with hirudin (1 Ulml). However, the addition of either anti-thrombin Ill or hirudin simultaneously with a-thrombin to the cultured endothelial monolayers did not prevent neutrophil adherence. The monoclonal antibody MoAb 60.3, which precipitates a complex of four neutrophil surface glycoproteins (CDv18) was used to further characterize the reaction. MoAb 60.3 decreased the thrombin-induced adherence of neutrophils to the endothelial mono-

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