Chemiluminescence response and adherence of neutrophils to cultured endothelial cells-influence of immunoglobulin G

✍ Scribed by Jonas, Eleonore ;Dwenger, Alexander ;Jonas, Michael

• Publisher: John Wiley and Sons
• Year: 1995
• Weight: 402 KB
• Volume: 10
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170100305

✦ Synopsis

The effect of different concentrations (0.87,4.35,8.7,17.5, 25 and 35mg/mL) of intravenous immunoglobulin G (Endobulin@) on neutrophil-endothelial cell interaction was studied using an in vitro model of human umbilical cord vein endothelial cells and human neutrophils. Because adherence of neutrophils t o endothelial cells is an essential component in inflammatory processes leading t o endothelial cell injury the influence of immunoglobulin G on adherence has been investigated.

A second aim of the present study was t o determine changes in chemiluminescence response of neutrophils during adherence t o endothelial cells. Production of oxygenderived metabolites, measured by chemi I uminescence response of neutrophi Is, decreased significantly in the presence of 8.7 mg immunoglobulin/mL test during coincubation of neutrophils and endothelial cells (p < 0.025). The adherence of neutrophils t o endothelial cells was significantly decreased at a concentration of 8.7mg immunoglobulin/mL test (p < 0.025).

The present results indicate that this preparation of immunoglobulin G might exert a protective effect on neutrophil-endothelial cell interaction by decreasing adherence of neutrophils t o endothelial cells and by scavenging reactive oxygen metabolites. Therefore, the current investigation points t o a probable protective effect of immunoglobulin G in oxidative diseases, such as the adult respiratory distress syndrome.