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Three-phase partitioning of protease from Calotropis procera latex

✍ Scribed by Saroat Rawdkuen; Phanuphong Chaiwut; Punyawatt Pintathong; Soottawat Benjakul


Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
375 KB
Volume
50
Category
Article
ISSN
1369-703X

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Osmotin purified from the latex of Calot
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A protein, similar to osmotin- and thaumatin-like proteins, was purified from Calotropis procera (Ait.) R.Br latex. The isolation procedure required two cation exchange chromatography steps on 50mM Na-acetate buffer (pH 5.0) CM-Sepharose Fast Flow and 25 mM Na-phosphate buffer (pH 6.0) Resource-S, r