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Procerain, a Stable Cysteine Protease from the Latex of Calotropis procera.

✍ Scribed by Vikash Kumar Dubey; M. V. Jagannadham


Publisher
John Wiley and Sons
Year
2003
Weight
4 KB
Volume
34
Category
Article
ISSN
0931-7597

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A protein, similar to osmotin- and thaumatin-like proteins, was purified from Calotropis procera (Ait.) R.Br latex. The isolation procedure required two cation exchange chromatography steps on 50mM Na-acetate buffer (pH 5.0) CM-Sepharose Fast Flow and 25 mM Na-phosphate buffer (pH 6.0) Resource-S, r